Plant is sessile as it is constantly being exposed to environment stresses. These stresses can cause the build-up of reactive oxygen species (ROS) in plant cell that will cause oxidative damage on the DNA, lipid and also proteins. There are two types of oxidative modified protein, one being reversible and another one could lead to permanent damage to the plant cell. The reversible proteins also usually act as signalling proteins. Hence, detection and identification of these proteins is prime target for crop improvement.  In this study, 5-Iodoacetamidofluorescein (5’IAF) that competes with hydrogen peroxide was used to identify oxidative modified cysteine proteins following the application of oxidative stress. This approach enables the identification of six groups of proteins that regulate the plant stress mechanisms. The six groups of proteins include L-ascorbate peroxidase redox protein, S-adenosylmethionine synthase and cysteine synthase that regulate amino acid metabolism, salt stress root protein RS1, photosynthesis proteins and seed storage proteins. The interaction of the key proteins observed in this study with the downstream of metabolites in combination with other phenotypes will provide a full picture of the role of these proteins in stress adaptation. The findings provide useful information as to production of stress tolerance plant in the future.