Improvements in 2D gel electrophoresis for biomarker discovery in plasma proteins — ASN Events
  1. Schedule
  2. Lunch & Poster Session One
  3. Improvements in 2D gel electrophoresis for biomarker discovery in plasma proteins

Improvements in 2D gel electrophoresis for biomarker discovery in plasma proteins (#112)

Stuart Brown 1 , Gillian Norris 1
  1. Massey University, Palmerston North, NZ, New Zealand
Publish consent withheld
  1. Omenn, G.S., THE HUPO human plasma Proteome project. Proteomics Clinical Applications, 2007. 1(8): p. 769-779.
  2. Li, X.H., et al., Comparison of alternative analytical techniques for the characterisation of the human serum proteome in HUPO Plasma Proteome Project. Proteomics, 2005. 5(13): p. 3423-3441.
  3. Rabilloud, T., Two-dimensional gel electrophoresis in proteomics: Old, old fashioned, but it still climbs up the mountains. Proteomics, 2002. 2(1): p. 3-10.
  4. Kim, M.R. and C.W. Kim, Human blood plasma preparation for two-dimensional gel electrophoresis. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2007. 849(1-2): p. 203-210.
  5. Penno, M., M. Ernst, and P. Hoffmann, Comparative 2D DIGE analysis of the depleted serum proteome, in Methods in molecular biology 854; Difference gel electrophoresis (DIGE), methods and protocols, R. Cramer and R. Westermeier, Editors. 2012, Springer: London. p. 207-220.
  6. Wu, H.C., et al., Isoelectric Focusing Management: An Investigation for Salt Interference and an Algorithm for Optimization. Journal of Proteome Research, 2010. 9(11): p. 5542-5556.
  7. Schmidt, A.C., B. Storr, and N.A. Kummer, Influence of one- and two-dimensional gel electrophoresis procedure on metal-protein bindings examined by electrospray ionization mass spectrometry, inductively coupled plasma mass spectrometry, and ultrafiltration. Talanta, 2011. 85(2): p. 1118-1128.