As the field of proteomics research has evolved over the past ten years, from identification focused experiments to today’s highly quantitative strategies, different techniques have been developed to meet the workflow needs. Most recently, SWATH™ Acquisition, a data independent acquisition workflow, has been developed that increases the comprehensiveness of data collection while maintaining high quantitative reproducibility. Now, thousands of proteins can be profiled across large sample sets with high data completeness and quantitative accuracy approaching that of the gold standard MRM approach. In DIA, larger width Q1 windows are stepped across the mass range in an LC timescale, transmitting populations of peptides for fragmentation, and high resolution MS and MS/MS spectra are acquired.  Post-acquisition, MS/MS data is interrogated using spectral libraries to generate quantitative XICs, providing MRM-like data for better specificity and quantitative accuracy.  Significant progress has been made in understanding and improving the analytical attributes of this DIA technique, using key sample types. For a new technique to have impact on biomarker research however, it must be routinely implemented across multiple labs. Progress to date on the use of DIA workflows for biomarker research will be discussed.