In this era, proteomics is one of the omics research fields that are voraciously studied in order to understand more on gene expression and genome annotation. Rice has played a very prominent role over centuries as a staple food and nutrient provider consumed by more than half of world populations. Even though there are many studies being carried out for wetland rice protein identification, none has been reported for upland rice. This preliminary study aims to help establish the proteome profile of Malaysian upland rice in the hope that it would be beneficial in understanding the proteins with diverse functions and predicting function of unknown proteins. In this study protein from upland rice was extracted by using TCA/Acetone based method. The protein pattern was compared on 1D SDS-PAGE, the excised gel bands and total protein were digested with trypsin and analyzed by Liquid chromatography-tandem mass spectrometry (LC-MS/MS). The MS/MS spectra obtained were subjected to search against Oryza sativa database. Comparison of the protein on 1D SDS-PAGE showed similar pattern for all cultivars. However, analysis via mass spectrometry revealed that the same protein presented may not be detected in all cultivars. More than 200 proteins have been identified and most of the proteins found are responsible for the growth and development of the plant, stress tolerance, antioxidant and natural sweetener. This finding gives insightful information with regards to the various proteins expressed and synthesized in upland rice that could contribute to a better understanding of its physiological significance. It could bring us one step closer to determine the function of every gene in upland rice particularly the one that is closely related with its growth and development.