Rice (Oryza sativa L.) is immensely consumed by global population and becoming the staple food in many countries. Providing a lot of nutrient content, rice comes in various variety, shapes, color and taste. Typical rice breeding is in flooded condition with good irrigation system referred to as wetland variety hence, requiring substantial amount of land. Our focus is on varieties that can sustain drought condition and require minimal amount of water for its growth known as upland rice. Upland rice in Malaysia is mostly grown either on hilly side or low land area without subjecting the plants to flooded condition where the source of water comes from rainfall. We set out to analyze a few of Malaysian upland rice varieties to identify the proteins to establish seed proteome database for rice from Malaysia. Protein were extracted from the seeds using suitable method adapted from Singh et al (2004) followed by separation on 1D SDS-PAGE, tryptic digestion and peptide sequencing by LC-MS/MS. Identified proteins were then searched against Oryza sativa databases. We confirmed the presence of water stress inducible protein which confers the upland variety tolerance to drought aside from many other proteins such as storage proteins, embryo-specific proteins, stress-related proteins, signaling proteins and several antioxidants. Responses to stress, biotic and abiotic stress, and protein metabolism are the largest representation of GO analysis. Our findings presents a preliminary insight into the seed proteome of Malaysian upland rice which could provide an alternative to typical rice breeding due to land scarcity.