Matrix Assisted Laser Desorption Ionisation Imaging Mass Spectrometry (MALDI-IMS) is a robust technique that has seen great improvement in recent time and has the potential to further the field of diagnostic imaging. However with automated sample preparation approaches becoming more and more prevalent, little work is being done to understand and standardize the critical matrix application and co-crystallisation techniques in use, or investigate more economical non-machine based approaches. Our research has led us to dissect and define the conditions responsible for the successful implementation of a sublimation based approach for sample preparation. By quantifying each condition and empirically determining their ideal conditions, we propose a repeatable and cost effective method for the universal MS imaging of Formalin fixed paraffin embedded (FFPE) tissue. In this work, we have demonstrated a universal tissue preparation and matrix application technique with low startup costs that can be operated by “walk-up” users and provide explanation and background information for further customization and optimization by experienced and inexperienced operators alike. We as also describe a novel technique for a non-delocalising method of Trypsin application. The application of such a technique has also opened up new avenues of research that allow for the combination of disparate methodologies to incorporate IMS as a standard indicative technique; We are aiming for the integration of IMS as a standard operating protocol in the same way other techniques have become ubiquitous in the lab. Only through the dissemination of complete and unabridged protocols and the empirical determinations for the conditions that govern them, will integration of disparate techniques create workflows with global perspective and therefore global results.